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S2255

Safranin O

Dye content ≥85 %, Powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
477-73-6
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899516
EC Number:
207-518-8
MDL number:
MFCD00011759
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
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Product Name

Safranin O, Dye content ≥85 %

form

powder

Quality Level

200

composition

Dye content, ≥85%

technique(s)

microbe id | staining: suitable

pH

10 (20 °C, 10 g/L)

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

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General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT3086
0000312792
0000312792
SHBS2699
SHBR8784

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Classic Papers in Orthopaedics (2014)
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Yufeng Zhang
Biomaterials (2012)
Paul A. Banaszkiewicz
Classic Papers in Orthopaedics (2014)
Maria D
Introduction to Diagnostic Microbiology for the Laboratory Sciences (2014)
A new modification and application of the Gram stain
Hucker GJ
Journal of Bacteriology, 6(4), 395-395 (1921)
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Global Trade Item Number

SKUGTIN
S2255-100G04061836918552
S2255-25G04061836918576
S2255-5G04061834990680

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