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Merck
CN

S3306

Sabouraud dextrose broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

SDB, Sabouraud Glucose Broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607

Key Documents

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agency

according to EP, according to JP, according to USP

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

Dextrose, 20.00 g/L , Mycological Peptone, 10.00 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pkg of -100 g, pkg of -500 g

storage condition

(Dry place, tightly sealed containers)

technique(s)

microbiological culture: suitable

color

light yellow

pH

5.6±0.2 (25 °C)

application(s)

microbiology, bioburden testing
cleaning products
cosmetics
food and beverages
personal care

storage temp.

2-25°C

suitability

selective by low pH for Aspergillus spp., selective by low pH for Candida spp., selective by low pH for Penicillium spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for aciduric bacteria, selective by low pH for molds (General Media), selective by low pH for yeasts (General Media)

Quality Level

200

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General description

Sabouraud Dextrose Broth is useful for cultivating fungi associated with skin infections. Mycological peptone provides nitrogenous compounds, and dextrose is an energy source. The low pH favors fungal growth and inhibits contaminating bacteria.

Application

SabouraudDextrose Broth is recommended for the selective cultivation of yeasts, molds,and aciduric bacteria. The medium is particularlyuseful for the cultivation of fungi associated with skin infections. It is alsosuitable for the microbiological examination of non-sterile products (accordingto USP, EP, and JP).

Preparation Note

Suspend 30 g of Sabouraud Dextrose Broth in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure. (121°C) for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH6708
BCCG0988
BCCF0766
BCCD0282
BCCB3594

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Muhammad Shahid et al.
Frontiers in cellular and infection microbiology, 10, 31-31 (2020-03-03)
Prototheca bovis (formerly P. zopfii genotype-II) is an opportunistic, achlorophyllous alga that causes mastitis in cows and skin disease in cats and dogs, as well as cutaneous lesions in both immunocompetent and immunosuppressed humans. Antifungal medications are commonly ineffective. This
Sabouraud, K.
Ann. Dermatol. Syphilol., 3, 1061-1061 (1892)
Drake Phelps et al.
Scientific reports, 7(1), 11244-11244 (2017-09-13)
Changes in resident microbiota may have wide-ranging effects on human health. We investigated whether early life microbial disruption alters neurodevelopment and behavior in larval zebrafish. Conventionally colonized, axenic, and axenic larvae colonized at 1 day post fertilization (dpf) were evaluated using
Tara R Catron et al.
Scientific reports, 9(1), 7064-7064 (2019-05-10)
Estrogenic chemicals are widespread environmental contaminants associated with diverse health and ecological effects. During early vertebrate development, estrogen receptor signaling is critical for many different physiologic responses, including nervous system function. Recently, host-associated microbiota have been shown to influence neurodevelopment.
Shabir Ahmad Lone et al.
BMC complementary medicine and therapies, 20(1), 131-131 (2020-05-01)
The global prevalence of fungal diseases is increasing rapidly, which affects more than a billion people every year with significant mortality rate. On the other hand, the development of new drugs to treat these fungal infections is slow, while the

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