SBR00025
Novobiocin
Ready Made Solution, 100 mg/mL in water
Synonym(s):
Novobiocin
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About This Item
Linear Formula:
C31H35N2NaO11
Molecular Weight:
634.61
NACRES:
NA.76
UNSPSC Code:
51286504
biological source
microbial
Quality Level
assay
≥90%
form
liquid, ready-to-use solution
concentration
100 mg/mL in water
color
faint yellow to dark brown
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria, mycobacteria, neoplastics
mode of action
DNA synthesis | interferes, enzyme | inhibits
storage temp.
−20°C
Related Categories
General description
Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.
Application
Novobiocin has been used:
- in cell culture systems to treat mycoplasma contaminations.[3]
- in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]
Biochem/physiol Actions
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II
Activity spectrum: Active against gram-positive and gram-negative bacteria
Activity spectrum: Active against gram-positive and gram-negative bacteria
Features and Benefits
- Ready available solution reduce the need for preparation time
- Commonly used in Cell Biology and Biochemical applications
- High quality antibiotic suitable for mulitple research applications
Preparation Note
Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.
Other Notes
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
涉药品监管产品
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Environmentally constrained mutation and adaptive evolution in Salmonella.
Massey, R. C., Rainey, P. B., Sheehan, B. J., Keane, O. M., & Dorman, C. J.
Current Biology, 9, 1477-1481 (1999)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)
Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth.
Hooper, D. C., Wolfson, J. S., McHugh, G. L., Winters, M. B., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 22, 662-671 (1982)
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
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