SE100
Silver Enhancer Kit
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About This Item
NACRES:
NA.47
UNSPSC Code:
12171500
form
liquid
Quality Segment
usage
sufficient for ≥100 slides staining and fixing
dilution
(for the determine the presence of gold nanorods in various tissues)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Application
Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.
Biochem/physiol Actions
Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.
Features and Benefits
Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.
Packaging
The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.
Kit Components Only
Product No.
Description
- Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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