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Merck
CN

SE100

Silver Enhancer Kit

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About This Item

NACRES:
NA.47
UNSPSC Code:
12171500
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form

liquid

Quality Level

usage

sufficient for ≥100 slides staining and fixing

dilution

(for the determine the presence of gold nanorods in various tissues)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Biochem/physiol Actions

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.


Kit Components Only

Product No.
Description

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Silver enhancer solution A 100 mL
    SDS

  • Silver enhancer solution B 100 mL
    SDS

pictograms

Health hazardEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
SE100-1KT04061832344409