T1438
Todd Hewitt broth
NutriSelect® Plus, powder, nonselective for Streptococcus spp., pkg of 100 g, pkg of 500 g
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Ph:
7.8±0.2 (25 °C)
Form:
powder
Suitability:
nonselective for Streptococcus spp.
Application(s):
clinical testing
food and beverages
microbiology
food and beverages
microbiology
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
packaging
pkg of 100 g, pkg of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
pH
7.8±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
storage temp.
2-25°C
suitability
nonselective for Streptococcus spp.
General description
Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.
Application
Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.
Recommended for the cultivation of group A hemolytic Streptococci used for serological studies.
Preparation Note
Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Other Notes
Components (g/L):
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type N95 (US)
Regulatory Information
动物来源培养基
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Articles
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
链球菌 — 检测、鉴定、分化和培养技术概述
Related Content
Alcamo's Laboratory Fundamentals of Microbiology (2010)
Handbook of Microbiological Media Third Edition (2004)
Finegold, S.M., et al.
Bailey and Scott's Diagnostic Microbiology (1982)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1438-100G | 04061834191117 |
| T1438-500G | 04061837337451 |