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T4375

Tetrazolium Blue Chloride

Name: Tetrazolium Blue Chloride
Brand: SIAL

used in colorimetric determination of reducing compounds

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Synonym(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

Empirical Formula (Hill Notation):

C₄₀H₃₂Cl₂N₈O₂

CAS Number:

1871-22-3

Molecular Weight:

727.64

Beilstein:

3894077

EC Number:

217-488-8

MDL number:

MFCD00040933

PubChem Substance ID:

24900207

Quality Level

200

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Hematology Stains

Histology Stains

Application

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H350

Precautionary Statements

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Preparation of a new tetrazolium salt which yields a blue pigment on reduction and its use in the demonstration of enzymes in normal and neoplastic tissues.

A M RUTENBURG et al.

Cancer research, 10(2), 113-121 (1950-02-01)

Endothelins-1/3 and endothelin-A/B receptors expressing glial cells with special reference to activated microglia in experimentally induced cerebral ischemia in the adult rats.

J J Li et al.

Neuroscience, 167(3), 665-677 (2010-03-09)

We reported previously that amoeboid microglial cells (AMC) in the developing brain exhibited endothelins (ETs) expression which diminished with advancing age and was undetected in microglia in the more mature brain. This study sought to explore if microglia in the

Sensitive colorimetric assay of carbidopa and methyldopa using tetrazolium blue chloride in pharmaceutical products.

Issopoulos, P.B.

International Journal of Clinical Pharmacy, 11, 213-217 (1989)

[Development of a novel Francisella tularensis antigen stained with tetrazolium-blue for tularemia microagglutination test].

Celebi B

Mikrobiyoloji bulteni (2013)

Ternary complex of molybdenum(VI) with 4-nitrocatechol and tetrazolium blue chloride and Its application to extraction-spectrophotometric analysis of ferrous metallurgy products.

Dimitrov., A.N., et al.

Journal of Analytical Chemistry, 62, 122-125 (2007)

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