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T8907

Millipore

Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect® Basic, powder, suitable for microbiology

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Synonym(s):
Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth
MDL number:
MFCD00132536
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

quality

Microbiologically tested.

packaging

poly bottle of 1 kg
poly bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

application(s)

environmental
food and beverages
pathogen testing
pharmaceutical
microbiology

suitability

bacteria

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General description

Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy.

Application

Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples.

Components

Casein (pancreatic digest) 17 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Ali Keleş et al.
Archives of microbiology, 203(1), 269-277 (2020-09-13)
Bacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model
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The Journal of experimental biology, 220(Pt 2), 322-327 (2016-11-05)
Stress-induced effects on innate immune activity in wild birds have been difficult to predict. These difficulties may arise from the frequent assumptions that (1) the stress response influences different components of the immune response similarly, (2) stress-induced effects do not
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Immuno-compromised patients such as those undergoing cancer chemotherapy are susceptible to bacterial infections leading to biofilm matrix formation. This surrounding biofilm matrix acts as a diffusion barrier that binds up antibiotics and antibodies, promoting resistance to treatment. Developing non-invasive imaging
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