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T9410

Millipore

Tryptone

Pancreatic digest of casein, Suitable for microbiology

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Synonym(s):
Peptone from casein, Peptone from casein, casein tryptic digested
CAS Number:
91079-40-2
EC Number:
293-428-4
NACRES:
NA.85

biological source

bovine milk

Quality Level

200

form

powder

packaging

poly bottle of 1 kg
poly bottle of 250 g

nitrogen analysis

11.4-13.9% total

loss

≤11% loss on drying

pH

6.6-7.4

application(s)

microbiology

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General description

Tryptone is a general-purpose growth peptone produced by the digestion of casein with the protease trypsin and used as a nitrogen source in culture media formulated for isolating and cultivating fastidious and non-fastidious bacteria, fungi, and some protozoa. It is a refined hydrolysate with high solubility and is used as a microbiological nutrient in laboratory media and fermentations. It is a high-quality source of amino acids and peptides produced by the pancreatic digestion of casein.

Application

Tryptone is recommended for laboratory media and fermentation and has been used in antibiotics, toxins, and enzyme productions. It is also used as a nutrient in vaccine production where a high free amino acid content is required. Tryptone may also be used in STGG (skim milk, tryptone, glucose, glycerol) medium at -80°C, which is used to preserve Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates for at least 3 years.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

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M B Hall et al.
Journal of dairy science, 99(1), 245-257 (2015-11-26)
Fructans are an important nonfiber carbohydrate in cool season grasses. Their fermentation by ruminal microbes is not well described, though such information is needed to understand their nutritional value to ruminants. Our objective was to compare kinetics and product formation
M B Hall
Journal of dairy science, 100(4), 2739-2750 (2017-02-22)
The availability of rumen-degradable protein (RDP) changes the use of carbohydrates by ruminal microbes. However, the effects of RDP on the simultaneous use of carbohydrate and formation of microbial products are not well described, although such information is needed to
S Oh et al.
Applied and environmental microbiology, 61(11), 3809-3814 (1995-11-01)
This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology.
Frederick K Balagaddé et al.
Science (New York, N.Y.), 309(5731), 137-140 (2005-07-05)
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying
S Gargallo et al.
Journal of dairy science, 103(7), 6209-6217 (2020-04-26)
Current feeding systems are based on the assumption that the AA profile of rumen undegraded protein is similar to that of the original feed. The objective of this experiment was to determine rumen bacterial degradation of individual essential AA in
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