Ultra pure 10x Taq Buffer with MgCl2

Ultra pure 10x Taq Buffer with MgCl2 is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. Ultra pure 10x Taq Buffer with MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase and Ultra pure DNA-free Water, to minimize false positive and negative results and improved DNA amplification.
Ultra pure 10x Taq Buffer with MgCl2 was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Ultra pure 10x Taq Buffer with MgCl2 can be used for:

• Quantitative PCR
• Amplification of complex genomic DNA and cDNA templates
• Amplification of low-copy number targets
• Microbiome analysis
• For further manufacture

Ultra pure 10x Taq Buffer with MgCl2 is provided in larger SKU sizes for ease of use in high throughput PCR applications and for further manufacture. Ultra pure 10x Taq Buffer with MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase and Ultra pure DNA-free Water to minimize false positive and negative results.

Why Choose Our Ultra pure & DNA-Free 10x Taq Buffer with MgCl₂?
When it comes to PCR-based workflows in regulated and high-sensitivity environments, the quality of your buffer is just as critical as the quality of your polymerase. Our Ultra pure & DNA-Free 10x Taq Buffer with MgCl₂ is engineered to deliver consistent, contamination-free performance across a broad range of PCR applications.

• Ultra pure & DNA-free-

• Our Ultra pure 10x Taq Buffer with MgCl₂ is manufactured under stringent conditions and rigorously tested to ensure the absence of detectable levels of DNA, DNase, RNase, and Nickase.
• Optimized for High-Sensitivity Applications- Whether you are working with low-copy number targets, complex genomic DNA, cDNA templates, or microbiome samples, our buffer is formulated to support reliable and sensitive amplification. Its inclusion of MgCl₂ provides the magnesium ions essential for Taq DNA polymerase activity, ensuring optimal enzyme performance straight from the vial with no additional supplementation required.
• Designed for Regulated Industries- This product was developed specifically for use in highly regulated industries where performance, reproducibility, and regulatory compliance are non-negotiable. It meets the demands of diagnostic assay development, further manufacture, and high-throughput quantitative PCR, making it a suitable choice for both research and quality-controlled production environments.
• The M-Clarity Program- Our Ultra Pure 10x Taq Buffer with MgCl₂ is backed by the M-Clarity Program, which provides full transparency across product quality, regulatory compliance, change notification, and documentation. This means you can select and use our products with confidence, knowing that any changes are communicated proactively and that full supporting documentation is available when you need it.
• Seamless Compatibility with the Ultra pure Ecosystem- Our 10x Taq Buffer with MgCl₂ is fully compatible with Ultra Pure JumpStart^™ Taq DNA Polymerase (UPJSTAQ) and Ultra pure DNA-Free Water (UPH2O), forming part of a complete, matched reagent system. Using matched Ultra pure reagents across your workflow minimizes the risk of false positive and false negative results and ensures that every component in your reaction meets the same rigorous purity standard.
• Ready for High-Throughput & Further Manufacture- Supplied in larger SKU sizes, our 10x Taq Buffer with MgCl₂ is designed with scalability in mind. Whether you are running high-throughput quantitative PCR in a clinical or industrial setting or incorporating it into a larger manufacturing process, our buffer delivers the consistency and volume you need to operate efficiently and reliably at scale.

Quality testing ensures the absence of detectable levels of contaminating DNA (<=1 copy of prokaryotic gDNA, <= 0.1 copy of eukaryotic gDNA, and <=10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

JumpStart is a trademark of Sigma-Aldrich Co. LLC