UPTQBF1
Ultra pure 10x Taq Buffer with MgCl2
DNA-free, Nuclease-free 10x PCR reaction buffer with MgCl2, designed for further manufacture
form
liquid
feature
DNA free, RNase and DNase free (also Nickase free)
packaging
pkg of 1.5 mL
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
Ultra pure 10x Taq Buffer with MgCl2 is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. Ultra pure 10x Taq Buffer with MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart™ Taq DNA Polymerase and Ultra pure DNA-free Water, to minimize false positive and negative results and improved DNA amplification.
Ultra pure 10x Taq Buffer with MgCl2 was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.
Ultra pure 10x Taq Buffer with MgCl2 was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.
Application
Ultra pure 10x Taq Buffer with MgCl2 can be used for:
- Quantitative PCR
- Amplification of complex genomic DNA and cDNA templates
- Amplification of low-copy number targets
- Microbiome analysis
- For further manufacture
Features and Benefits
- DNA-free
- DNase-free, RNase-free, and nickase-free
- Ideal for high throughput quantitative PCR applications
- Compatible with Ultra pure JumpStart™ Taq DNA Polymerase
- Enhanced change control and quality agreement
Packaging
Ultra pure 10x Taq Buffer with MgCl2 is provided in larger SKU sizes for ease of use in high throughput PCR applications and for further manufacture. Ultra pure 10x Taq Buffer with MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart™ Taq DNA Polymerase and Ultra pure DNA-free Water to minimize false positive and negative results.
Other Notes
Quality testing ensures the absence of detectable levels of contaminating DNA (<=1 copy of prokaryotic gDNA, <= 0.1 copy of eukaryotic gDNA, and <=10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Discover Ultra pure PCR Solutions for reliable molecular diagnostics with high-quality RT-PCR reagents and JumpStart™ Taq DNA Polymerase for accurate results.
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