W2261
WL nutrient broth
suitable for microbiology, NutriSelect® Plus
Synonym(s):
Wallerstein Laboratory Nutrient Broth
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171607
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
5.5±0.2 (25 °C)
application(s)
bioburden testing
food and beverages, microbiology
suitability
selective for Candida spp., selective for Escherichia coli, selective for Lactobacillus spp., selective for Pichia spp., selective for Proteus spp., selective for Saccharomyces spp., selective for Zygosaccharomyces spp., selective for coliforms
Application
Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations.
Preparation Note
Suspend 60.25 g of WL Nutrient Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. If desired to obtain a pH of 6.5 add a 1% solution of sodium bicarbonate (Cat. No. 71627).
Other Notes
Ingredients (g/L)
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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