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X100PC

Sigma-Aldrich

Triton™ X-100

peroxide- and carbonyl-free

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Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
Beilstein:
2315025
MDL number:
MFCD00128254
PubChem Substance ID:
329830773

description

non-ionic

Quality Level

200

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

packaging

ampule of 5, 5x5, 10x5 mL

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

0·3% Triton™ X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

Packaged in ampules under Argon.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

H302 - H315 - H318 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model.
American Journal of Translational Research, 8(2), 799-799 (2016)
Cytotoxicity of bilirubin for human fibroblasts and rat astrocytes in culture. Effect of the ratio of bilirubin to serum albumin.
Chuniaud L, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 256(2), 103-114 (1996)
Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.
Experimental Eye Research, 79(1), 131-140 (2004)
The size, shape, and hydration of nonionic surfactant micelles. Triton X-100.
Robson RJ and Dennis EA
The Journal of Physical Chemistry, 81, 1075-1078 (1977)
Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM).
Koley D and Bard AJ
Proceedings of the National Academy of Sciences of the USA, 107, 16783-16783 (2010)
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