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01494

Nancy-520

Name: Nancy-520
Brand: SIGMA

suitable for RT-PCR, ≥90.0% (HPCE)

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About This Item

UNSPSC Code:

12171500

EC Number:

200-664-3

NACRES:

NA.32

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Quality Level

100

assay

≥90.0% (HPCE)

form

liquid

mol wt

78.13 g/mol

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

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color

red to very dark red

bp

189 °C/1,013 hPa

mp

18.4 °C

solubility

DMSO: soluble

density

1.104 g/cm³ at 20 °C

fluorescence

λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA

greener alternative category

Aligned,

storage temp.

2-8°C

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Greener Alternative Products

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - Solvent

flash_point_c

87 °C - closed cup - Solvent

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Nolan, T. and Bustin, S.A.

PCR Technology: Current Innovations (2013)

Rodriguez-Lazaro, D.

Real-Time PCR in Food Science: Current Technology and Applications (2013)

Park, D.J.

PCR Protocols (2011)

Wilks, M.

PCR Detection of Microbial Pathogens (2012)

Filion, M.

Quantitative Real-time PCR in Applied Microbiology (2012)

View All Related Papers

Articles

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

定量PCR基础知识

实时聚合酶链式反应可用于计算样本的起始材料量。它相比常规PCR具有更大的检测动态范围。

Global Trade Item Number

SKU	GTIN
CDS007614-500MG	04061828988471
01494-50UL	04061837731846
01494-10ML	04061838609427
01494-500UL	04061837731839
01494-5ML	04061838609434

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