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01632

Atto 520-Biotin

Name: Atto 520-Biotin
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Biotin-Atto 520

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About This Item

Empirical Formula (Hill Notation):

C₃₇H₅₃ClN₆O₈S

Molecular Weight:

777.37

UNSPSC Code:

12352108

eCl@ss:

34058011

PubChem Substance ID:

329747336

NACRES:

NA.32

MDL number:

MFCD04040671

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product line

BioReagent

assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble, ethanol: soluble, methanol: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 522-528 nm A_max

suitability

suitable for fluorescence

storage temp.

2-8°C

InChI

1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;

InChI key

OCYRRKMPTKPPHX-QITHAKLPSA-N

Application

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Interaction of Escherichia coli RNA polymerase σ70 subunit with promoter elements in the context of free σ70, RNA polymerase holoenzyme, and the β'-σ70 complex.

Vladimir Mekler et al.

The Journal of biological chemistry, 286(1), 270-279 (2010-10-19)

Promoter recognition by RNA polymerase is a key point in gene expression and a target of regulation. Bacterial RNA polymerase binds promoters in the form of the holoenzyme, with the σ specificity subunit being primarily responsible for promoter recognition. Free

Detection of vimentin serine phosphorylation by multicolor Quenchbodies.

Hee-Jin Jeong et al.

Biosensors & bioelectronics, 40(1), 17-23 (2012-07-17)

Protein phosphorylation is a key event in intracellular signal transduction, and fluorescent biosensor for the specific phosphorylation event in a target protein is considered highly useful as a tool of cellular biology and drug screening. Vimentin, the most abundant intermediate

Single-molecule fluorescence studies reveal long-range electron-transfer dynamics through double-stranded DNA.

Manoj Kumbhakar et al.

Chemphyschem : a European journal of chemical physics and physical chemistry, 10(4), 629-633 (2009-01-30)

Complex dynamics of combined long-range fluorescence resonance energy transfer (FRET) and short-range electron transfer (ET) processes in a single double-stranded DNA (dsDNA) molecule (see figure) reveals that FRET remains almost unaltered in the presence of ET. Present systems also demonstrate

Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples.

Siddharth Nanguneri et al.

PloS one, 7(5), e38098-e38098 (2012-06-05)

Three-dimensional fluorescence imaging of thick tissue samples with near-molecular resolution remains a fundamental challenge in the life sciences. To tackle this, we developed tomoSTORM, an approach combining single-molecule localization-based super-resolution microscopy with array tomography of structurally intact brain tissue. Consecutive

Protein-labeling effects in confocal laser scanning microscopy.

Christopher A Teske et al.

The journal of physical chemistry. B, 109(28), 13811-13817 (2006-07-21)

Confocal laser scanning microscopy (CLSM) is being increasingly used for observing protein uptake in porous chromatography resins. Recent CLSM studies have revealed the possible existence of a nondiffusive protein transport mechanism. Observing protein uptake with CLSM requires labeling the protein

Articles

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Global Trade Item Number

SKU	GTIN
E35702-5G	04061832621227
01632-1MG-F	04061823353137

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