02744
Atto 655-Streptavidin
BioReagent, suitable for fluorescence
Synonym(s):
Streptavidin-Atto 655
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352116
product line
BioReagent
Quality Level
form
powder
manufacturer/tradename
ATTO-TEC GmbH
solubility
DMF: soluble, DMSO: soluble, acetonitrile: soluble
fluorescence
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
suitability
suitable for fluorescence, suitable for gel electrophoresis
storage temp.
−20°C
Application
Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.
Analysis Note
free of unconjugated dye
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)
Articles
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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