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About This Item
Empirical Formula (Hill Notation):
C6Fe2KN6 · xH2O
CAS Number:
Molecular Weight:
306.89 (anhydrous basis)
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
InChI
1S/6CN.2Fe.K/c6*1-2;;;/q;;;;;;-3;+2;+1
InChI key
ZJSIHHBKYVAMOE-UHFFFAOYSA-N
form
powder
solubility
water: 0.1 g/10 mL, blue to very deep blue
εmax
≥120 at 253-263 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Application
Prussian blue is not a dye but an inorganic pigment containing ferric ferrocyanide. Procedures have been published for the demonstration of non-hemoglobin, cytoplasmic iron, according to Sundberg and Broman, and for the demonstration of hemosiderin according to Highman and from Lille and Fullmer based the work originally done by Perle in 1867. Prussian Blue is formed in situ in both of these methods. In combination with iron particles such as superparamagnetic iron oxide nanoparticles, Prussian blue may be used for tracking of transplanted cells such as mesenchymal stem cells. Prussian blue may also be used to differentiate slow versus fast cycling cells by methods that monitor iron oxide particle labeling dilution thru cell division.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A new modification of Perls's reaction for hemosiderin in tissues.
Highman, B.
Archives of Pathology, 33, 937-938 (1942)
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Nachweis von eisenoxyd in Gewissen pigmenten
Virchow?s Arch. (Pathol. Anat.), 39, 42-48 (1867)
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Magn. Reson. in Med., 66, 1362-1373 (2011)
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