07985
6-Aminofluorescein
BioReagent, suitable for fluorescence, ~95% (HPLC)
Synonym(s):
Fluoresceinamine isomer II
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About This Item
Empirical Formula (Hill Notation):
C20H13NO5
CAS Number:
Molecular Weight:
347.32
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
257-334-7
MDL number:
Beilstein/REAXYS Number:
51708
product line
BioReagent
Quality Segment
assay
~95% (HPLC)
form
powder
impurities
≤5% 5-aminofluorescein
mp
285 °C (dec.) (lit.)
solubility
DMSO: soluble, acetone: soluble, methanol: soluble
fluorescence
λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
suitability
suitable for fluorescence
SMILES string
Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
InChI
1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
InChI key
YOAWSYSKQHLFPM-UHFFFAOYSA-N
Application
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.
Other Notes
Note: Do not confuse with fluorescamine.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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