biological source
human neonatal foreskin or adult skin (normal)
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 46
morphology
Fibroblast
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
General description
Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Cell Line Origin
Application
Components
Preparation Note
- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture Routine
Disclaimer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Protocols
This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
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