Key Documents

View All Documentation

10820

Aprotinin from bovine lung

Name: Aprotinin from bovine lung
Brand: SIGMA

lyophilized, ~80% (HPCE), crystalline (fine), white, ≥3500 U/mg

Synonym(s):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₈₄H₄₃₂N₈₄O₇₉S₇

CAS Number:

9087-70-1

Molecular Weight:

6511.44

UNSPSC Code:

12352202

NACRES:

NA.26

EC Number:

232-994-9

MDL number:

MFCD00130541

eCl@ss:

32190102

Form:

crystalline (fine)

Assay:

~80% (HPCE)

Biological source:

bovine lung

Mol wt:

~6,500

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

biological source

bovine lung

Quality Level

200

assay

~80% (HPCE)

form

crystalline (fine)

quality

lyophilized

specific activity

≥3500 U/mg

mol wt

~6,500

technique(s)

electrophoresis: suitable

color

white

solubility

phosphate buffer: 5 mg/mL, clear, colorless (PH 7.6 (70 mm phosphate))

UniProt accession no.

P00974

storage temp.

2-8°C

SMILES string

S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O

InChI

1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Gene Information

cow ... PTI(404172)

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Other Notes

1 U corresponds to 1 biological kallikrein inhibitor unit (KIU)

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品

低风险生物材料

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Immunoblotting for Calpastatin Expression.

Monica Averna et al.

Methods in molecular biology (Clifton, N.J.), 1915, 195-206 (2019-01-09)

Immunoblotting is a procedure routinely used to analyze calpastatin expression. However, immunoblotting alone may not be adequate for this task, since calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications. Here we

Drop-on-demand cell bioprinting via Laser Induced Side Transfer (LIST).

Hamid Ebrahimi Orimi et al.

Scientific reports, 10(1), 9730-9730 (2020-06-18)

We introduced and validated a drop-on-demand method to print cells. The method uses low energy nanosecond laser (wavelength: 532 nm) pulses to generate a transient microbubble at the distal end of a glass microcapillary supplied with bio-ink. Microbubble expansion results in

Production and Purification of Recombinant Calpastatin.

Roberta De Tullio et al.

Methods in molecular biology (Clifton, N.J.), 1915, 163-185 (2019-01-09)

The production of recombinant calpastatin in E. coli has become an efficient tool to obtain discrete amounts of a specific calpastatin species that can be present concomitantly with other calpastatin fragments/forms in the same tissue or cell type in a

Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells.

Elizabeth J Osterlund et al.

Science advances, 8(16), eabm7375-eabm7375 (2022-04-21)

Cytoplasmic and membrane-bound BCL-2 family proteins regulate apoptosis, a form of programmed cell death, via dozens of binary protein interactions confounding measurement of the effects of inhibitors in live cells. In cancer, apoptosis is frequently dysregulated, and cell survival depends

Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly.

Axel Himmelbach et al.

Bio-protocol, 8(15), e2955-e2955 (2018-08-05)

Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture

View All Related Papers

Global Trade Item Number

SKU	GTIN
10820-100MG	04061838679567
10820-5MG	04061838679574
10820-25MG	04061833616031

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service