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10831

Histopaque®-1083

sterile-filtered, density: 1.083

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.083

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Biochem/physiol Actions

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Features and Benefits

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL4897
RNBL5440
RNBK9776
RNBK6510
RNBK4686

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Bella S Guerrouahen et al.
Pediatric blood & cancer, 53(6), 1132-1135 (2009-06-02)
Tyrosine kinase inhibitors, such as imatinib, have dramatically improved the outcomes for patients with selected cancers. For imatinib, western blotting of phospho-CrkL was an insensitive, indirect, and descriptive method to determine drug efficacy. Greater use of targeted therapies should involve
Gangduo Wang et al.
Toxicology and applied pharmacology, 237(2), 188-195 (2009-04-01)
Even though reactive oxygen and nitrogen species (RONS) are implicated as mediators of autoimmune diseases (ADs), little is known about contribution of protein oxidation (carbonylation and nitration) in the pathogenesis of such diseases. The focus of this study was, therefore
Qiyue Hu et al.
Scientific reports, 8(1), 7675-7675 (2018-05-18)
Interleukin-15 (IL-15) can promote both innate and adaptive immune reactions by stimulating CD8+/CD4+ T cells and natural killer cells (NK) while showing no effect in activating T-regulatory (Treg) cells or inducing activation-associated death among effector T cells and NK cells.
Ahmad J H Albaghdadi et al.
Scientific reports, 9(1), 6528-6528 (2019-04-27)
Polycystic ovary syndrome (PCOS) is characterized by failure of ovulation and is associated with obesity and chronic inflammation. Recent evidence suggests that anomalous activation of ovarian macrophages and numerical and functional deficits in the Th17 (CD4+IL17A+) and the CD4+CD25+CD127low Tregs
The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.
Andreas Brown et al.
Haematologica, 105(2), 317-324 (2019-05-11)
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Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

默克密度梯度介质_细胞分离-默克生命科学

我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。

Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

Global Trade Item Number

SKUGTIN
39075-250MG-F04061832808826
294721-1G04061826601457
39075-50MG-F04061832808833
10831-6X100ML04061838682826
10831-100ML04061838682673

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