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11557

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

Synonym(s):

AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

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About This Item

Empirical Formula (Hill Notation):
C18H24N6O6S4
CAS Number:
30931-67-0
Molecular Weight:
548.68
NACRES:
NA.83
PubChem Substance ID:
329749485
UNSPSC Code:
12352204
EC Number:
250-396-6
MDL number:
MFCD00010404
Beilstein/REAXYS Number:
7329461
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Product Name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

Quality Segment

200

assay

≥98% (HPLC)

form

powder or crystals

impurities

≤6.0% water

solubility

H2O: 10 mg/mL, slightly hazy to clear

storage temp.

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:
  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts
  • to test the antioxidant capacity (AOC) of Aloe vera samples
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Other Notes

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

wgk

WGK 2

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