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14340C

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

protein free, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD01634638
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Quality Level

400

description

for research or for further manufacturing use, without L-glutamine

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: 3.45 g/L
L-glutamine: no
NaHCO3: 1.6 g/L
HEPES: 7.5 mM
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL™ 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media (Catalog No. 14324C and 14326C) can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™, and DHFR- selection systems).

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number
0000456486
0000456487
0000456487
0000456486
RNBL3354

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Sapan J Patel et al.
Electrophoresis, 38(3-4), 417-428 (2016-11-03)
The mechanisms underlying the aberrant growth and interactions between cells are not understood very well. The pre-B acute lymphoblastic leukemia cells directly obtained from an adult patient grow very poorly or do not grow at all at low density (LD)

Articles

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Global Trade Item Number

SKUGTIN
14340C-1000ML04061838734822
14340C-500ML04061837383076

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