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Sigma-Aldrich

BES

BioXtra, for molecular biology, ≥99.5% (T)

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Synonym(s):
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
Beilstein:
1781572
EC Number:
233-465-5
MDL number:
MFCD00007533
PubChem Substance ID:
57647223

grade

for molecular biology

product line

BioXtra

Assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue (900 °C)

≤0.05%

pH

2.5-5.0 (25 °C, 1 M in H2O)

useful pH range

6.4-7.8

pKa (25 °C)

7.1

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.095
λ: 280 nm Amax: 0.080

SMILES string

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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Application

BES, or N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, can be used to study molecular biology, biological buffers, molecular biology reagents and zwitterionic compounds. BES has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES has also been used to determine that saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Marina V Kirillova et al.
Inorganic chemistry, 51(9), 5224-5234 (2012-04-10)
The facile aqueous medium reactions of copper(II) nitrate with BES biobuffer [(HOCH(2)CH(2))(2)N(CH(2)CH(2)SO(3)H), hereinafter referred as H(3)bes] in the presence of various benzenecarboxylic acids [benzoic (Hba), 3-hydroxybenzoic (Hhba), and 3,5-dihydroxybenzoic (Hdhba) acid] and lithium hydroxide gave rise to the self-assembly generation
Yingjie Zhang et al.
Nano letters, 15(7), 4657-4663 (2015-06-24)
Noncrystalline semiconductor materials often exhibit hysteresis in charge transport measurements whose mechanism is largely unknown. Here we study the dynamics of charge injection and transport in PbS quantum dot (QD) monolayers in a field effect transistor (FET). Using Kelvin probe
John A Ormiston et al.
EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology, 10(12), 1449-1455 (2014-11-26)
Stent fracture is important because it may cause adverse events. The interventional cardiologist needs independent data to aid stent selection. Stent designers need data to improve stent design. We used a repetitive bend test to compare durability and fracture for
Brian A Karolewski et al.
Human gene therapy, 14(14), 1287-1296 (2003-09-25)
A number of different transfection reagents have been used for lentiviral vector production. We directly compared transfection buffers, DNA purification methods, chemical facilitators, and DNA concentrations to optimize production. The use of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), sodium butyrate, and one
Yingjie Zhang et al.
ACS nano, 9(10), 10445-10452 (2015-09-25)
Artificial solids composed of semiconductor quantum dots (QDs) are being developed for large-area electronic and optoelectronic applications, but these materials often have defect-induced in-gap states (IGS) of unknown chemical origin. Here we performed scanning probe based spectroscopic analysis and density
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