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Merck
CN

16555

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside

≥98.0% (HPLC), powder or crystals

Synonym(s):

X-α-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
107021-38-5
Molecular Weight:
408.63
UNSPSC Code:
12352204
PubChem Substance ID:
329751267
NACRES:
NA.32
MDL number:
MFCD00063780

Key Documents

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Product Name

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside, ≥98.0% (HPLC)

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI key

OPIFSICVWOWJMJ-YGEXGZRRSA-N

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14+/m1/s1

assay

≥98.0% (HPLC)

form

powder or crystals

optical activity

[α]/D 135.0±5.0°, c = 1 in DMF/H2O 1:1

storage temp.

−20°C

Quality Level

100

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Application

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside is suitable as a substrate for α-galactosidase. It has been used:
  • in the yeast two-hybrid screen
  • for differentiating α-galactosidase-positive strains of yeast
  • to determine melibiase activity in yeast and to identify Saccharomyces cerevisiae A241 × Saccharomyces uvarum C995 hybrids

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN3177
BCCK2011
BCCG2043
BCCG2042
BCCB0971

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Tubb, R.S. and Liljestrom, P.L.
Journal of the Institute of Brewing, 92, 588-588 (1986)
A novel reporter gene MEL1 for the yeast two-hybrid system.
S Aho et al.
Analytical biochemistry, 253(2), 270-272 (1997-12-31)
A Unique Saccharomyces cerevisiae ? Saccharomyces uvarum Hybrid Isolated From Norwegian Farmhouse Beer: Characterization and Reconstruction
Krogerus K, et al.
Frontiers in Microbiology, 9 (2018)
Jiaojie Li et al.
Cells, 8(12) (2019-11-28)
Amyotrophic lateral sclerosis (ALS) caused by mutation of superoxide dismutase 1 (SOD1), affects various cellular processes and results in the death of motor neurons with fatal defects. Currently, several neurological disorders associated with DNA damage are known to directly induce
Florence Teulé et al.
Nature protocols, 4(3), 341-355 (2009-02-21)
The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This protocol describes silk

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