16805
Atto 425 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
Atto 425-N-hydroxysuccinimide ester
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About This Item
Empirical Formula (Hill Notation):
C26H30N2O8
CAS Number:
Molecular Weight:
498.53
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:
Quality Segment
product line
BioReagent
assay
≥90% (HPLC), ≥90% (degree of coupling)
manufacturer/tradename
ATTO-TEC GmbH
solubility
DMF: soluble, DMSO: soluble, H2O: soluble
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 422-428 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O
InChI
1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
InChI key
PUEQEMDTFPYCDY-UHFFFAOYSA-N
General description
Atto 425 is a member of the commercially available coumarin derivatives.
Application
Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Other Notes
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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