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17490

Bromocresol Purple

suitable for microscopy

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
115-40-2
Molecular Weight:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57647609
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
MFCD00011681
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form

powder

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

mp

240 °C (dec.) (lit.)

solubility

hot ethanol: 0.1 g/10 mL

εmax

≥400 at 414-424 nm in methanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Application

Spectrophotometric determination of serum-albumin

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCC6412
BCCB5311
BCBZ4065
BCBV9386
BCBV0503

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Yeast colonies synchronise their growth and development
Palkova Z and Forstova J
Journal of Cell Science, 113(11), 1923-1928 (2000)
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity
Kurzweilova H and Sigler K
Yeast, 9(11), 1207-1211 (1993)
C H White et al.
Journal of dairy science, 89(7), 2459-2464 (2006-06-15)
The MicroFoss method was evaluated for its effectiveness as an indicator of fluid milk shelf life. Half-gallon, 2% fat fluid milk samples (n = 90) were obtained from a milk processing plant on 3 occasions postpasteurization and evaluated for shelf
André Klauss et al.
Photosynthesis research, 102(2-3), 499-509 (2009-03-31)
Determination of thermodynamic parameters of water oxidation at the photosystem II (PSII) manganese complex is a major challenge. Photothermal beam deflection (PBD) spectroscopy determines enthalpy changes (ΔH) and apparent volume changes which are coupled with electron transfer in the S-state
Annalise E Zemlin et al.
Nutrition (Burbank, Los Angeles County, Calif.), 25(10), 1006-1010 (2009-06-03)
Hypoalbuminemia is known to occur in critically ill patients and is associated with increased mortality. Severe hypoalbuminemia is defined in the literature as serum albumin levels lower than 24 g/L. Albumin levels were measured in our laboratory using the bromocresol
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Global Trade Item Number

SKUGTIN
642673-5G04061833357170
642673-1G04061832729336
17490-5G04061838752154
17490-10G04061838752147
17490-50G04061835570324

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