sufficient for 500 tests
not available in USA
UV/Vis spectroscopy: suitable
corresponds for UV test (WST reagent, WST formazan)
use tested
2-8°C
Danger
Resp. Sens. 1
10 - Combustible liquids
WGK 3
Not applicable
Not applicable
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
How to Find the Product Number
Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).
Example:
Additional examples:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
Having trouble? Feel free to contact Technical Service for assistance.
How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
This kit contains WST Solution (1x5mL), Enzyme Solution (1x100μL), Buffer Solution (1x100mL), Dilution Buffer (1x50mL), and a manual.
The equipment needed to use this kit is a plate reader (450 nm filter), a 96-well microplate, 10 μL & 100-200 μL pipettes, a multichannel pipette and an incubator. Superoxide dismutase (SOD) is also necessary for the preparation of an inhibition curve.
Dilute 1mL of WST Solution with 19mL of Buffer Solution.
Centrifuge the Enzyme Solution tube for five seconds. Mix by pipeting, and dilute 15μL of Enzyme Solution with 2.5mL of Dilution Buffer.
Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows: 200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.
1. J. M. McCord and I. Fridovich, J. Biol. Chem., 244, 6049 (1969). 2. S. Goldstein and G. Czapski, Free Rad. Res. Comms.,12-13(Part 1), 5-10 (1991). 3. R. H. Burdon, V. Gill, and C. Rice-Evans, Free Rad. Res. Comms., 18, 369 (1993). 4. M. W. Sutherland and B. Learmonth, Free Rad. Res., 27, 283 (1997). 5. H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999). 6. H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, Biosci. Biotechnol. Biochem., 63, 485 (1999). 7. A. V. Peskin, et al., Clin. Chim. Acta., 293, 157 (2000).
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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