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19362

4-Methylumbelliferyl butyrate

Name: 4-Methylumbelliferyl butyrate
Brand: SIGMA

lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence

Synonym(s):

MU-Bu

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₄O₄

CAS Number:

17695-46-4

Molecular Weight:

246.26

NACRES:

NA.32

PubChem Substance ID:

57647789

UNSPSC Code:

12352204

EC Number:

241-694-7

MDL number:

MFCD00038087

Beilstein/REAXYS Number:

227973

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Properties

Product Name

4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)

Quality Level

100

assay

≥95% (HPCE)

form

powder or crystals

mp

90-92 °C (lit.)

solubility

DMF: soluble, DMSO: soluble

fluorescence

λ_ex 312 nm in methanol, λ_ex 360 nm; λ_em 449 nm (Reaction product), λ_ex 365 nm; λ_em 445 nm in 0.1 M Tris pH 8.0 (Lipase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

InChI key

WKPUJZVCZXWKCK-UHFFFAOYSA-N

Description

Application

suitable as fluorogenic substrate for esterases/lipases

Other Notes

Fluorogenic substrate for butyrate esterase

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Determination of lipase activity using image analysis.

Marcelo Müller-Santos et al.

Analytical biochemistry, 351(2), 305-307 (2006-03-10)

Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate.

M Vaneechoutte et al.

Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)

Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5

Comparison of three rapid methods, tributyrine, 4-methylumbelliferyl butyrate, and indoxyl acetate, for rapid identification of Moraxella catarrhalis.

E Speeleveld et al.

Journal of clinical microbiology, 32(5), 1362-1363 (1994-05-01)

Moraxella catarrhalis can easily be differentiated from other oxidase-positive, gram-negative cocci with tributyrine, 4-methylumbelliferyl butyrate, or indoxyl acetate. All M. catarrhalis give positive reactions, and all Neisseria spp. give negative reactions. The 4-methylumbelliferyl butyrate tube test and indoxyl acetate strip

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Global Trade Item Number

SKU	GTIN
19362-1G	04061838760869
19362-5G	04061838760876