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Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

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Synonym(s):
5(6)-Carboxy-di-O-acetylfluorescein
Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
124387-19-5
Molecular Weight:
460.39
MDL number:
MFCD00467494
PubChem Substance ID:
329752214
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90.0% (HPLC)

form

powder

solubility

DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6

InChI

1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)

InChI key

SWRGCNMDGRMQGB-UHFFFAOYSA-N

Related Categories

General description

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Application

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R E Petroski et al.
Journal of neuroscience methods, 52(1), 23-32 (1994-04-01)
A method for selectively labeling cultured neurons using the vital dye, 5(6)-carboxyfluorescein diacetate (CFDA), is described. This non-fluorescent membrane-permeant dye is cleaved by cytosolic esterases into the fluorescent anion, 5(6)-carboxyfluorescein (CF). Both astrocytes and neurons exhibit brilliant fluorochromasia within minutes
Ting Yang et al.
Immunobiology, 218(4), 548-553 (2012-08-14)
Donor lymphocyte transfusion (DLT) may induce the graft-versus-leukemia (GVL) effect for patients with AML relapsed after transplant. However, AML is a highly diverse disease and the limited overall efficacy of DLT in clinical practice emphasizes the importance of identifying a
Zhiyuan Zhang et al.
The Plant cell, 29(8), 2027-2046 (2017-07-28)
Plasmodesmata (PDs) play vital roles in cell-to-cell communication and plant development. Emerging evidence suggests that sterols are involved in PD activity during cytokinesis. However, whether sterols contribute to PD gating between established cells remains unknown. Here, we isolated
L C J Ellis et al.
Toxicology in vitro : an international journal published in association with BIBRA, 28(6), 1165-1175 (2014-06-08)
Hepatic efflux of drug candidates is an important issue in pre-clinical drug development. Here we utilise a method which quantifies and distinguishes efflux of drugs at the canalicular and sinusoidal membranes in rat hepatocyte cultures. Bi-phasic kinetics of transport of
S L Hempel et al.
Free radical biology & medicine, 27(1-2), 146-159 (1999-08-12)
To detect intracellular oxidant formation during reoxygenation of anoxic endothelium, the oxidant-sensing fluorescent probes, 2',7'-dichlorodihydrofluorescein diacetate, dihydrorhodamine 123, or 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate were added to human umbilical vein endothelial cells during reoxygenation. None of these fluorescent probes were able to
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