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Sigma-Aldrich

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

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Synonym(s):
Nitrate/Nitrite Assay Kit, colorimetric
NACRES:
NA.84

Quality Level

100

usage

sufficient for 100 assays

suitability

passes test for application

storage temp.

2-8°C

Related Categories

Application

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.

Kit Components Only

Product No.
Description
  • NaNO2 Standard Solution (100 mM) 1 bottle
  • NaNO3 Standard Solution (100 mM) 1 bottle
  • Buffer solution (20 mM, pH 7.6) 1 bottle
  • Nitrate reductase (lyophilized) 1 bottle
  • Enzyme Co-factor (lyophilized) 1 bottle
  • Griess Reagent A 1 bottle
  • Griess Reagent B 1 bottle

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Fredrik Sjövall et al.
PloS one, 9(5), e97673-e97673 (2014-05-16)
The levels of nitric oxide (NO) and various cytokines are known to be increased during sepsis. These signaling molecules could potentially act as regulators and underlie the enhancement of mitochondrial function described in the later phase of sepsis. Therefore, we
Mohamed Raâfet Ben Khedher et al.
Lipids in health and disease, 16(1), 241-241 (2017-12-14)
Vasculogenic erectile dysfunction (VED) is considered as a common complication among people with type 2 diabetes (T2D). We tested whether changes in fatty acid (FAs) classes measured in erythrocytes are associated with increased risk of diabetic VED along with related
Othman A Al-Shboul et al.
Biomedical reports, 9(6), 511-516 (2018-12-14)
Previous studies have shown that progesterone could inhibit muscle contraction in various sites of the gastrointestinal tract. The underlying mechanisms responsible for these inhibitory effects of progesterone are not fully known. The aim of the current study was to investigate
Othman A Al-Shboul et al.
Journal of pregnancy, 2019, 4302309-4302309 (2019-05-14)
It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the
Jolanta Zuwała-Jagiełło et al.
Clinical and experimental hepatology, 1(3), 105-111 (2015-11-01)
To determine plausible associations between liver cirrhosis and circulating endothelial cell-derived microparticles (EMPs), vascular endothelial growth factor (VEGF) levels and plasma nitric oxide (NO) metabolites. Sixty patients with cirrhosis and 20 healthy control subjects were enrolled in the study. Circulating
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