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28616

Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonym(s):

Biotin-Atto 425

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About This Item

Empirical Formula (Hill Notation):
C37H53N5O7S
Molecular Weight:
711.91
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
57648315
NACRES:
NA.32
MDL number:
MFCD04974818
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product line

BioReagent

assay

≥95.0% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O

InChI

1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)

InChI key

LPVYZZMUDCZDQO-UHFFFAOYSA-N

General description

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBT5953
BCBT0526
BCBR3434V
BCBQ2289V
BCBP1564V

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Articles

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution.
Praus, P., et al.
Spectroscopy: An International Journal, 24, 303-307 (2010)
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Global Trade Item Number

SKUGTIN
28616-1MG-F04061823353595