3017
Transcreener® EPIGEN Methyltransferase Assay
Sign Into View Organizational & Contract Pricing
Select a Size
About This Item
UNSPSC Code:
41122100
NACRES:
NA.84
shipped in
dry ice
storage temp.
−20°C
General description
The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.
View full Transcreener® product list
View full Transcreener® product list
Physical form
Kit with buffered aqueous solutions
Preparation Note
3017-1K = 1,000 assay, 384-well
3017-10K = 10,000 assay, 384-well
3017-10K = 10,000 assay, 384-well
Legal Information
Transcreener is a registered trademark of BellBrook Labs
Regulatory Information
低风险生物材料
常规特殊物品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service