Transcreener^® EPIGEN Methyltransferase Assay

The Transcreener^® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener^® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener^® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener^® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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Kit with buffered aqueous solutions

3017-1K = 1,000 assay, 384-well
3017-10K = 10,000 assay, 384-well

Transcreener is a registered trademark of BellBrook Labs