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Merck
CN

30326

EMPIGEN® BB detergent

~30% active substance

Synonym(s):

N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine

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About This Item

Linear Formula:
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
CAS Number:
66455-29-6
UNSPSC Code:
12161900
NACRES:
NA.25
PubChem Substance ID:
329753464
EC Number:
266-368-1
Beilstein/REAXYS Number:
3670807
MDL number:
MFCD00084742

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InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

InChI key

DVEKCXOJTLDBFE-UHFFFAOYSA-N

SMILES string

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

description

zwitterionic

form

liquid

composition

solid content, 35.0-45.0 % (w/v)

concentration

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

density

1.030-1.060 g/mL at 20 °C

UV absorption

λ: 417 nm Amax: ≤0.055, λ: 441 nm Amax: ≤0.037

Quality Level

200

Related Categories

Application

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses.

Legal Information

EMPIGEN is a registered trademark of Huntsman Corporation

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH5993
BCBQ6339V
BCBQ6339
BCBP8409

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Adam M Rotunda et al.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 30(7), 1001-1008 (2004-06-24)
Phosphatidylcholine injections are becoming an increasingly popular technique to treat localized fat accumulation. This formula is composed primarily of phosphatidylcholine and sodium deoxycholate, a bile salt used to solubilize the natural phospholipid in water. The mechanism through which this injectable
R Jennings et al.
Journal of medical virology, 31(2), 98-108 (1990-06-01)
HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal
M Lukácová et al.
European journal of epidemiology, 10(2), 227-230 (1994-04-01)
A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates
Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
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