30483
Anti-Mouse IgG−Abberior® CAGE 635 antibody produced in goat
for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
Synonym(s):
Abberior® CAGE 635-Anti-Mouse-IgG antibody produced in goat
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About This Item
UNSPSC Code:
12352200
biological source
goat
Quality Segment
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
availability
available only in USA and Canada
concentration
~1 mg/mL
fluorescence
λex 630 nm; λem 653 nm in PBS, pH 7.4 (photoactivated)
storage temp.
−20°C
General description
Abberior CAGE 635 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent red dye.
It can most effectively be excited from 620 to 645 nm. Abberior CAGE 635 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm.
Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 630 (photoactivated, PBS pH 7.4), 316 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 653 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,75 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (photoactivated, PBS pH 7.4)
It can most effectively be excited from 620 to 645 nm. Abberior CAGE 635 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm.
Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 630 (photoactivated, PBS pH 7.4), 316 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 653 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,75 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (photoactivated, PBS pH 7.4)
Application
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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