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32670

4′,6-Diamidino-2-phenylindole dihydrochloride

Name: 4′,6-Diamidino-2-phenylindole dihydrochloride
Brand: SIGMA

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

Synonym(s):

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₅N₅ · 2HCl

CAS Number:

28718-90-3

Molecular Weight:

350.25

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57648699

EC Number:

249-186-7

Beilstein/REAXYS Number:

4894417

MDL number:

MFCD00012681

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product line

BioReagent

Quality Level

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, H₂O: soluble

fluorescence

λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex), λ_ex 374 nm; λ_em 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

suitability

suitable for fluorescence

storage temp.

room temp

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

GHS07

signalword

Warning

hcodes

H315,H317,H335

pcodes

P261 - P264 - P271 - P272 - P280 - P302 + P352

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Rhs proteins from diverse bacteria mediate intercellular competition.

Sanna Koskiniemi et al.

Proceedings of the National Academy of Sciences of the United States of America, 110(17), 7032-7037 (2013-04-11)

Rearrangement hotspot (Rhs) and related YD-peptide repeat proteins are widely distributed in bacteria and eukaryotes, but their functions are poorly understood. Here, we show that Gram-negative Rhs proteins and the distantly related wall-associated protein A (WapA) from Gram-positive bacteria mediate

Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection.

Angus T Stock et al.

The Journal of experimental medicine, 211(5), 751-759 (2014-04-23)

Infection induces the expression of inflammatory chemokines that recruit immune cells to the site of inflammation. Whereas tissues such as the intestine and skin express unique chemokines during homeostasis, whether different tissues express distinct chemokine profiles during inflammation remains unclear.

Autographa californica multiple nucleopolyhedrovirus core gene ac96 encodes a per Os infectivity factor (PIF-4).

Minggang Fang et al.

Journal of virology, 83(23), 12569-12578 (2009-09-18)

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac96 is a core gene, but its role in virus replication is still unknown. To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac96-null virus (vAc(96)(null)).

Neuronal ER stress impedes myeloid-cell-induced vascular regeneration through IRE1α degradation of netrin-1.

François Binet et al.

Cell metabolism, 17(3), 353-371 (2013-03-12)

In stroke and proliferative retinopathy, despite hypoxia driven angiogenesis, delayed revascularization of ischemic tissue aggravates the loss of neuronal function. What hinders vascular regrowth in the ischemic central nervous system remains largely unknown. Using the ischemic retina as a model

Turn-on, fluorescent nuclear stains with live cell compatibility.

Demar R G Pitter et al.

Organic letters, 15(6), 1330-1333 (2013-03-07)

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and

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Global Trade Item Number

SKU	GTIN
32670-25MG-F	04061826716380
32670-5MG-F	04061826716397

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