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Sigma-Aldrich

Eosin Methylene blue

according to May-Grünwald

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Colour Index Number:
881/1038
Colour Index Number:
45380/52015
MDL number:
MFCD00005038
PubChem Substance ID:
329754361
NACRES:
MA.02

form

powder or crystals

Quality Level

200

quality

according to May-Grünwald

εmax

≥1000 at 635-655 nm in methanol
≥650 at 512-532 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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General description

Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.
Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Application

Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317 - H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Detection of carboxylic acid group in organic compounds with eosin, mercurochrome, and methylene blue.
Johar GS.
Fresenius' Zeitschrift fur Analytische Chemie, 260 (2), 122-123 (1972)
The effect of concentration of dyes on differentiation of enteric bacteria on eosin-methylene-blue agar.
Journal of Bacteriology, 45 (5), 471-471 (1943)
Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane: a reconsideration of the specificity of the annexin V/propidium iodide assay.
Lecoeur H
Cytometry, 44 (1), 65-72 (2001)

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