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32884

Giemsa Stain

Modified Solution

Synonym(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

CAS Number:
51811-82-6
UNSPSC Code:
12171500
PubChem Substance ID:
329754377
NACRES:
MA.02
MDL number:
MFCD00081642
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Product Name

Giemsa Stain, Modified Solution, according to Giemsa

Quality Segment

form

liquid

quality

according to Giemsa

technique(s)

UV/Vis spectroscopy: suitable

color

dark blue

pH

6.9

density

1.036 g/cm3 at 20 °C

suitability

passes test for UV spectrum

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Biochem/physiol Actions

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

WGK

WGK 2

Flash Point (°F)

51.8 °F - closed cup

Flash Point (°C)

11 °C - closed cup

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