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Merck
CN

36801

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

Synonym(s):

N-Succinimidyl 7-(diethylamino)coumarin-3-carboxylate

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About This Item

Empirical Formula (Hill Notation):
C18H18N2O6
CAS Number:
139346-57-9
Molecular Weight:
358.35
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
57649664
MDL number:
MFCD00467585
Beilstein/REAXYS Number:
5359433

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Product Name

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester, BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

InChI

1S/C18H18N2O6/c1-3-19(4-2)12-6-5-11-9-13(17(23)25-14(11)10-12)18(24)26-20-15(21)7-8-16(20)22/h5-6,9-10H,3-4,7-8H2,1-2H3

SMILES string

CCN(CC)c1ccc2C=C(C(=O)ON3C(=O)CCC3=O)C(=O)Oc2c1

InChI key

NUNPVRICKDZFLK-UHFFFAOYSA-N

product line

BioReagent

assay

≥96.0% (HPLC)

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

fluorescence

λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for coupling to amines
suitable for fluorescence

storage temp.

−20°C

Quality Level

200

Application

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester is used as a reagent to conjugate 7-(diethylamino)coumarin-3-carboxylic acid (7-DCCA) to other molecules such as amino acids via amide chemistry. 7-DCCA is used to label amino acids prior to resolution by capillary zone electrophoresis. 7-DCCA is used as a reference in screening of nerve agent degradation products by MALDI-TOFMS.

Other Notes

Labeling of amino acids prior to resolution by capillary zone electrophoresis

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK6570
BCCK7429
BCCH7545
BCCD0021
BCCC7900

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You-Ren Shu et al.
Analytical chemistry, 78(13), 4697-4701 (2006-07-01)
A novel method for the rapid screening of degradation products derived from nerve agents by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. Five standard products were selected as model compounds, including isopropyl methylphosphonic acid (IMPA), pinacolyl methylphosphonic acid
T. Higashijima et al.
Analytical Chemistry, 64, 711-711 (1992)

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