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Merck
CN

37291

Dihydroethidium

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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About This Item

Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
104821-25-2
Molecular Weight:
315.41
UNSPSC Code:
12161505
NACRES:
NA.32
PubChem Substance ID:
57649715
MDL number:
MFCD00077335
Beilstein/REAXYS Number:
5107482

Key Documents

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Product Name

Dihydroethidium, BioReagent, suitable for fluorescence, ≥95% (HPCE)

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

product line

BioReagent

assay

≥95% (HPCE)

form

solid

Quality Level

100

mol wt

315.41 g/mol

solubility

acetonitrile: soluble
methanol: soluble

fluorescence

λex 358 nm; λem 461 nm
λex 392 nm; λem 410 nm in methanol

suitability

suitable for fluorescence

storage temp.

−20°C

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Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.

Other Notes

B-ring reduced analog of ethidium

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF0204
BCCD2348
BCBZ9421
BCBZ9279
BCBV6252

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The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(9), 4011-4023 (2013-03-01)
This study addresses the relationship between cochlear oxidative damage and auditory cortical injury in a rat model of repeated noise exposure. To test the effect of increased antioxidant defenses, a water-soluble coenzyme Q10 analog (Qter) was used. We analyzed auditory
Francisco R M Laurindo et al.
Methods in enzymology, 441, 237-260 (2008-06-17)
Assessment of low-level superoxide in nonphagocytic cells is crucial for assessing redox-dependent signaling pathways and the role of enzymes such as the NADPH oxidase complex. However, most superoxide probes present inherent limitations. Particularly, assessment of dihydroethidium (DHE) fluorescence is limited
Karina A Roth et al.
Journal of endodontics, 38(9), 1253-1256 (2012-08-16)
Root canal fillings are intended to prevent microbial proliferation over time in the canal after treatment. The objective of this study was to assess biofilm proliferation within the sealer-dentin interfaces of 2 methacrylate resin-based systems, self-etch (SE) and total-etch (TE)
Hai-Yan Jin et al.
PloS one, 7(6), e38502-e38502 (2012-06-14)
Inflammation and oxidative stress play a crucial role in angiotensin (Ang) II-mediated vascular injury. Angiotensin-converting enzyme 2 (ACE2) has recently been identified as a specific Ang II-degrading enzyme but its role in vascular biology remains elusive. We hypothesized that loss
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