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Dihydroethidium

Name: Dihydroethidium
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

Empirical Formula (Hill Notation):

C₂₁H₂₁N₃

CAS Number:

104821-25-2

Molecular Weight:

315.41

Beilstein:

5107482

MDL number:

MFCD00077335

PubChem Substance ID:

57649715

NACRES:

NA.32

product line

BioReagent

Assay

≥95% (HPCE)

form

solid

solubility

acetonitrile: soluble
methanol: soluble

fluorescence

λ_ex 358 nm; λ_em 461 nm
λ_ex 392 nm; λ_em 410 nm in methanol

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

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Dry Dyes & Certified Stains

Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.

Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Linkage

B-ring reduced analog of ethidium

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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