37294
Dihydrofolate Reductase from bovine liver
~8 U/mL, ammonium sulfate suspension, off-white
Synonym(s):
Tetrahydrofolate Dehydrogenase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Dihydrofolate Reductase from bovine liver, ~8 U/mL, ammonium sulfate suspension, off-white
biological source
bovine liver
form
ammonium sulfate suspension
specific activity
≥6 U/mg protein
concentration
~8 U/mL
color
off-white
density
1.2 g/mL at 20 °C
storage temp.
2-8°C
Quality Level
Application
Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .
Biochem/physiol Actions
Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .
Other Notes
1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C
In folate metabolism for the synthesis of purines, thymidine and glycine
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Physical form
Suspension in 3.6 M ammonium sulfate solution, pH 7.0
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
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H B Eastman et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8572-8576 (1991-10-01)
Dihydrofolate reductase (DHFR; EC 1.5.1.3) is required in folate metabolism for the synthesis of purines, thymidine, and glycine. Although there have been several reports of induction of DHFR enzyme by methotrexate (MTX), a drug that competitively inhibits DHFR, there are
M A Pesce et al.
Clinical chemistry, 27(3), 380-384 (1981-03-01)
Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0
S P Chumakov et al.
Molekuliarnaia biologiia, 46(5), 699-711 (2012-11-20)
Association and degradation of protein complexes play essential role in a majority of normal and pathologic processes, which take place in living cell. Studying the underlying mechanisms of those interactions would give deeper understanding of specific causes of disease progression
Tetsuo Shimizu et al.
Anticancer research, 32(10), 4589-4596 (2012-10-13)
Pemetrexed inhibits three key folate enzymes: thymidylate synthetase (TYMS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT). The relationship between the clinical efficacy of pemetrexed and the expression of folate enzymes in lung cancer cells is unknown. The purpose of
Luca Belmonte et al.
Critical reviews in eukaryotic gene expression, 22(3), 219-232 (2012-11-13)
A state-of-the-art review of the role of the Langmuir-Blodgett nanotemplate on protein crystal structures is here presented. Crystals grown by nanostructured template appear more radiation resistant than the classical ones, even in the presence of a third-generation highly focused beam
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