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Sigma-Aldrich

Fluo 3-AM solution

1 mM in DMSO, ≥99.0% (TLC)

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Empirical Formula (Hill Notation):
C51H50Cl2N2O23
CAS Number:
121714-22-5
Molecular Weight:
1129.85
NACRES:
NA.32

Quality Level

100

Assay

≥99.0% (TLC)

form

liquid

concentration

1 mM in DMSO

fluorescence

λex 506 nm; λem 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase

storage temp.

−20°C

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General description

Cell permeable fluorescent indicator of intracellular Ca2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+; lower binding affinity allows measurement of higher peaks of Ca2+ transients than with Fura 2.

Application

suitable as fluorescent Ca sensitive probe (after cleavage of the AM ester)

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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