biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
concentration
~1 mg/mL
fluorescence
λex 635 nm; λem 655 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR 635 can substitute dyes like Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
Application
Abberior STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.
Suitability
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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