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41698

Atto 488 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 488

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352108
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product line

BioReagent

Quality Level

100

assay

≥90% (HPLC), ≥90% (degree of coupling)

form

powder

mol wt

686.66 g/mol

manufacturer/tradename

ATTO-TEC GmbH

color

orange, red

λmax

501-507 nm in methanol: water (1:1) (with 0.1% perchloric acid)

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-507 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CN(CCCC(=O)ON1C(=O)CCC1=O)C(=O)C2=CC=CC=C2C3=C4C=CC(=[NH2+])C(=C4OC5=C3C=CC(=C5S(=O)(=O)[O-])N)S(=O)(=O)O

InChI

InChI=1S/C29H26N4O12S2/c1-32(14-4-7-23(36)45-33-21(34)12-13-22(33)35)29(37)16-6-3-2-5-15(16)24-17-8-10-19(30)27(46(38,39)40)25(17)44-26-18(24)9-11-20(31)28(26)47(41,42)43/h2-3,5-6,8-11,30H,4,7,12-14,31H2,1H3,(H,38,39,40)(H,41,42,43)

InChI key

QYLVUXZLEBHDMK-UHFFFAOYSA-N

Related Categories

General description

Atto 488 NHS ester is a hydrophilic fluorescent label with high water solubility. The fluorescence activity is excited efficiently at the 480-515nm range. A suitable excitation source for Atto 488 is the 488 nm line of the Argon-Ion laser.

Application

Atto 488 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Features and Benefits

Characteristic features of the Atto 488 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP4899
BCCP4900
BCCP2429
BCCP3201
BCCP0744

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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
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Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
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Qian Peter Su et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15036-15046 (2020-06-17)
Mammalian DNA replication is initiated at numerous replication origins, which are clustered into thousands of replication domains (RDs) across the genome. However, it remains unclear whether the replication origins within each RD are activated stochastically or preferentially near certain chromatin
Kin Man Suen et al.
Nature communications, 11(1), 4242-4242 (2020-08-28)
Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle
Christiane Iserman et al.
Molecular cell, 80(6), 1078-1091 (2020-12-09)
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic elements under physiological buffer conditions and condensation is enhanced at human body temperatures (33°C and 37°C) and reduced
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Global Trade Item Number

SKUGTIN
41698-5MG-F04061832531991
41698-1MG-F04061832089980
41698-0.24MG-KC04061832521015
41698-50MG-F04061832531984
41698-5X0.24MG-KC04061832532004
41698-1VL-F04061832521022

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