Skip to Content
Merck
CN

41785

Dimidium bromide

BioReagent, suitable for fluorescence, ~95% (AT)

Synonym(s):

3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide, Trypadine, phenanthridinium 1553

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C20H18BrN3
CAS Number:
518-67-2
Molecular Weight:
380.28
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57650090
EC Number:
208-256-7
Beilstein/REAXYS Number:
3809779
MDL number:
MFCD00011757
Assay:
~95% (AT)
Form:
solid

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Dimidium bromide, BioReagent, suitable for fluorescence, ~95% (AT)

InChI key

MQOKYEROIFEEBH-UHFFFAOYSA-N

InChI

1S/C20H17N3.BrH/c1-23-19-12-15(22)8-10-17(19)16-9-7-14(21)11-18(16)20(23)13-5-3-2-4-6-13;/h2-12,22H,21H2,1H3;1H

SMILES string

[Br-].C[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

product line

BioReagent

assay

~95% (AT)

form

solid

Quality Level

100

mol wt

380.28 g/mol

concentration

80-100 % (w/w) (3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide)

color

dark purple to very dark purple
dark red to very dark red

mp

243-248 °C (lit.)

fluorescence

λex 306 nm; λem 612 nm in 50 mM Tris pH 8.0 (DNA)
λex 480 (pH 7.6, low ionic strength)

suitability

suitable for fluorescence

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Dimidium Bromide is used as a binding agent to natural DNA based on base-pair specificity. It is used as a fluorescence agent for nucleic acid experiments.
Intercalating probe for nucleic acids.

General description

Dimidium Bromide is a trypanocidal drug that is known to affect nucleic acid synthesis in many organisms e.g. tissue culture cells.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN1632
BCCL9621
BCCJ8750
BCCL0032
BCCH5955

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nuclear transplantation studies of the action of dimidium bromide on amoebae.
S E Hawkins et al.
Nature, 222(5188), 86-87 (1969-04-05)
G Dougherty
The International journal of biochemistry, 14(6), 493-504 (1982-01-01)
1. The absorption spectrum of three phenanthridine drugs (ethidium, dimidium and prothidium bromide) bound to natural DNAs of differing G-C content were obtained using a novel mixing scheme and analysed according to the excluded site binding model. 2. Ethidium bromide
Dimidium bromide or phenanthridinium 1553; a note on the present position.
J CARMICHAEL
The Veterinary record, 62(17), 257-257 (1950-04-29)
[Chemoprophylaxis of bovine trypanosomiasis T. congolense and T. vivax by phenathridinium S. 1553].
J DEOM
Annales de la Societe belge de medecine tropicale (1920), 30(1), 25-29 (1950-03-01)
T N Beliaeva et al.
Tsitologiia, 31(11), 1363-1368 (1989-11-01)
A natural DNA-intercalator plant benzo-c-phenanthridine alkaloid sanguinarine is more toxic for mouse transformed fibroblast L-cells in culture than synthetic DNA-intercalator ethidium bromide (EtB) and alkaloid berberine. Dimidium bromide is also an inhibitor of the L-cell growth. In assay conditions, growth
View All Related Papers

Related Content

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service