45245
Eosin 5-isothiocyanate
≥95.0% (UV)
Synonym(s):
EITC
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About This Item
Empirical Formula (Hill Notation):
C21H7Br4NO5S
CAS Number:
Molecular Weight:
704.96
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
assay
≥95.0% (UV)
fluorescence
λex 521; λem 544 (pH 9.0)
storage temp.
2-8°C
SMILES string
OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S
InChI
1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)
InChI key
MCXYFYWNOWPDMA-UHFFFAOYSA-N
Application
Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.
Other Notes
Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies
signalword
Danger
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
flash_point_f
Not applicable
hcodes
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Articles
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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S Q Liu et al.
The American journal of physiology, 264(5 Pt 1), C1155-C1164 (1993-05-01)
Although eosin-5-maleimide (EM) covalently labels band 3 and has been thought to react at the external-facing anion transport site, EM reversibly inhibits Cl- exchange at 0 degrees C in a noncompetitive fashion, indicating that under these conditions it does not
T Chiba et al.
Biochimica et biophysica acta, 858(1), 107-117 (1986-06-13)
The amino-acid residues of band 3 protein taking part in the ionic interaction with an anion-transport inhibitor, eosin 5-isothiocyanate (EITC), were determined by pH titration. The plots of the absorbance of EITC-ghost system against pH reveal five equilibria, at pH
T J Deerinck et al.
The Journal of cell biology, 126(4), 901-910 (1994-08-01)
A simple method is described for high-resolution light and electron microscopic immunolocalization of proteins in cells and tissues by immunofluorescence and subsequent photooxidation of diaminobenzidine tetrahydrochloride into an insoluble osmiophilic polymer. By using eosin as the fluorescent marker, a substantial