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Merck
CN

45253

Wright Stain solution

suitable for microscopy

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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About This Item

NACRES:
NA.47
PubChem Substance ID:
329756625
UNSPSC Code:
12171500
MDL number:
MFCD00005038

Key Documents

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Product Name

Wright Stain solution, suitable for microscopy

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

impurities

methanol

density

0.80 g/mL at 20 °C

suitability

suitable for microscopy
Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

Quality Level

100

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Application

Wright stain has been used to determine the number of white blood cell types (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) per microliter of whole blood.

General description

Wright Stain is a modified Romanowsky stain commonly used for the cellular elements of blood. Wright′s stain is a mixture of eosin Y with polychromed (oxidized and partly demethylated) methylene blue and therefore termed polychrome stains. The hematological stain is intended for differentially staining peripheral blood and bone marrow films.

Other Notes

Blood stain

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

target_organs

Eyes

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

51.8 °F

flash_point_c

11 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8135
BCCL6830
BCCH7368
BCCH9664
BCCG5575

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K. Leybold et al.
Praxis-Laboratorium: Grundlagen der Laboratoriumsdiagnostik, 24-24 (1976)
Analysis and testing of biological stains--the Biological Stain Commission Procedures.
Penney D P, et al.
Biotechnic & Histochemistry (2002)
Borrelia burgdorferi has minimal impact on the Lyme disease reservoir host Peromyscus leucopus.
Schwanz LE, et al.
Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 117-124 (2011)
Bernadette FR Jacqueline HC et al.
Clinical Hematology, 3rd Ed. Henry Kimpton (2015)
Young-Joo Jeong et al.
Anatomy & cell biology, 43(4), 294-302 (2011-01-27)
Asthma is a Th2-dependent disease mediated by IgE and Th2 cytokines, and asthmatic patients suffer from oxidative stresses from abnormal airway inflammation. Vitamin C is a micro-nutrient functioning as an antioxidant. When administered at a mega-dose, vitamin C has been

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