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Merck
CN

45260

Sigma-Aldrich

Eosin B

suitable for microscopy

Synonym(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
548-24-3
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein:
4121964
EC Number:
208-943-1
MDL number:
MFCD00005041
UNSPSC Code:
12171500
PubChem Substance ID:
329756626
NACRES:
NA.47

Key Documents

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technique(s)

titration: suitable

λmax

509-524 nm

suitability

suitable for microscopy

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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General description

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.

Application

Eosin B is an adsorption and fluorescent indicator in microscopy.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9334
BCCD7122
BCCD0415
BCCC9309
BCCC1582

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A A Waheed et al.
Analytical biochemistry, 287(1), 73-79 (2000-11-18)
Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than
Xu Wang et al.
Electrophoresis, 31(23-24), 3808-3815 (2010-11-17)
In this study, we describe an effective visualizing technique for proteins in SDS-PAGE based on the organic dye, Eosin B, the sensitivity of which can be further strengthened by the addition of magnesium to the staining solution after electrophoresis. The
Chloé E Atreya et al.
The Journal of biological chemistry, 278(16), 14092-14100 (2003-01-31)
Protozoal parasites are unusual in that their thymidylate synthase (TS) and dihydrofolate reductase (DHFR) enzymes exist on a single polypeptide. In an effort to probe the possibility of substrate channeling between the TS and DHFR active sites and to identify
Jishu Naskar et al.
Organic & biomolecular chemistry, 9(19), 6610-6615 (2011-08-13)
Self-assembling short peptides can offer an opportunity to make useful nano-/microstructures that find potential application in drug delivery. We report here the formation of multivesicular structures from self-assembling water-soluble synthetic amphiphilic dipeptides containing a glutamic acid residue at the C-terminus.
A A Waheed et al.
Journal of biochemical and biophysical methods, 33(3), 187-196 (1996-12-30)
We describe a detailed procedure for estimating a wide range of proteins by the eosin B dye method. At acidic pH, eosin B binds to proteins and absorption of the protein-dye complex at 536-544 nm is proportional to the concentration
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