All Photos(1)

Documents

46043

Ethidium homodimer

Name: Ethidium homodimer
Brand: SIGMA

suitable for fluorescence, ~90% (HPCE)

Sign Into View Organizational & Contract Pricing

All Photos(1)

Synonym(s):

EtDi, EthD-1

Empirical Formula (Hill Notation):

C₄₆H₅₀Cl₄N₈

CAS Number:

61926-22-5

Molecular Weight:

856.75

EC Number:

263-325-9

MDL number:

MFCD00210948

PubChem Substance ID:

329757072

Assay

~90% (HPCE)

Quality Level

100

form

solid

mp

≥250 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
H₂O: soluble

fluorescence

λ_ex 493 nm in H₂O
λ_ex 528 nm; λ_em 617 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Detection Substrates & Enzymes

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

Pictograms

GHS02,GHS05,GHS08

Signal Word

Danger

Hazard Statements

H261 - H314 - H351

Precautionary Statements

P231 + P232 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react 3

Storage Class Code

4.3 - Hazardous materials, which set free flammable gases upon contact with water

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

How to Find the Product Number

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503

Product Number

25G

Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

How to Find a Lot/Batch Number for COA

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Impact of Cell Composition and Geometry on Human Induced Pluripotent Stem Cells-Derived Engineered Cardiac Tissue.

Takeichiro Nakane et al.

Scientific reports, 7, 45641-45641 (2017-04-04)

The current study describes a scalable, porous large-format engineered cardiac tissue (LF-ECT) composed of human induced pluripotent stem cells (hiPSCs) derived multiple lineage cardiac cells with varied 3D geometries and cell densities developed towards the goal of scale-up for large

Sustained release of adipose-derived stem cells by thermosensitive chitosan/gelatin hydrogel for therapeutic angiogenesis.

Nai-Chen Cheng et al.

Acta biomaterialia, 51, 258-267 (2017-01-31)

Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is

Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids.

J Markovits et al.

Analytical biochemistry, 94(2), 259-264 (1979-04-15)

Adhesion-induced eosinophil cytolysis requires the receptor-interacting protein kinase 3 (RIPK3)-mixed lineage kinase-like (MLKL) signaling pathway, which is counterregulated by autophagy.

Susanne Radonjic-Hoesli et al.

The Journal of allergy and clinical immunology, 140(6), 1632-1642 (2017-04-17)

Eosinophils are a subset of granulocytes that can be involved in the pathogenesis of different diseases, including allergy. Their effector functions are closely linked to their cytotoxic granule proteins. Release takes place through several different mechanisms, one of which is

Endogenous viable cells in lyopreserved amnion retain differentiation potential and anti-fibrotic activity in vitro.

Yong Mao et al.

Acta biomaterialia, 94, 330-339 (2019-06-10)

Human amniotic membrane (AM) has intrinsic anti-inflammatory, anti-fibrotic and antimicrobial properties. Tissue preservation methods have helped to overcome the short shelf life of fresh AM allowing "on demand" use of AM grafts. Cryopreserved AM that retains all native tissue components

View All Related Papers