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47140

4-Fluoro-7-nitrobenzofurazan

suitable for fluorescence, BioReagent, ≥98.0% (HPLC)

Synonym(s):

NBD-F

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About This Item

Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
29270-56-2
Molecular Weight:
183.10
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
329757296
MDL number:
MFCD00010196
Beilstein/REAXYS Number:
1077571
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product line

BioReagent

Quality Level

200

assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λex 470 nm; λem 528 nm in ethanol, λex 470 nm; λem 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

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Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

235.4 °F - closed cup

flash_point_c

113 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9196

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Nasr Y Khalil
Talanta, 80(3), 1251-1256 (2009-12-17)
A fully automated, rapid and highly sensitive HPLC method with automated sample pre-treatment by column-switching system and fluorescence detection has been developed for the trace quantitative determination of the new antidepressant reboxetine (RBX) in human plasma. A simple pre-column derivatization
Yanting Song et al.
Amino acids, 42(5), 1897-1902 (2011-04-21)
A high-performance liquid chromatography method in which fluorescence detection is used for the simultaneous determination of 21 amino acids is proposed. Amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and then separated on a monolithic silica column (MonoClad C18-HS, 150 mm×3
Yang-Ki Park et al.
Journal of chromatography. A, 1217(20), 3357-3361 (2010-04-13)
Single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis by attaching a drop to the tip of a capillary. With a 2-layer drop comprised of an aqueous basic acceptor phase covered with a thin organic layer, acidic analytes
Chiaki Aoyama et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(1), 63-65 (2009-01-14)
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of
Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
View All Related Papers

Global Trade Item Number

SKUGTIN
47140-10MG04061833277317
47140-50MG04061833277324

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