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47671

Formamide

Name: Formamide
Brand: SIGMA

BioUltra, Molecular Biology, ≥99.5% (T)

Synonym(s):

Amide C₁, Formic amide

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About This Item

Linear Formula:

HCONH₂

CAS Number:

75-12-7

Molecular Weight:

45.04

UNSPSC Code:

12352111

NACRES:

NA.25

PubChem Substance ID:

57651075

EC Number:

200-842-0

Beilstein/REAXYS Number:

505995

MDL number:

MFCD00007941

Assay:

≥99.5% (T)

Grade:

Molecular Biology

Technique(s):

FISH: suitable, hybridization: suitable, protein extraction: suitable

Bp:

210 °C (lit.)

Vapor pressure:

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

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grade

Molecular Biology

Quality Level

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

product line

BioUltra

assay

≥99.5% (T)

form

liquid

autoignition temp.

932 °F

technique(s)

FISH: suitable, hybridization: suitable, protein extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.447

pH

7-9 (25 °C, 1 M in H₂O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

solubility

H₂O: 10 M at 20 °C, clear, colorless

density

1.134 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤1 mg/kg, sulfate (SO₄^2-): ≤10 mg/kg

SMILES string

NC=O

cation traces

Ag: ≤5 mg/kg, Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, NH₄⁺: ≤1000 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Tl: ≤5 mg/kg, Zn: ≤1 mg/kg

λ

10 M in H₂O

UV absorption

λ: 260 nm A_max: ≤0.4, λ: 280 nm A_max: ≤0.1

application(s)

genomic analysis

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Related Categories

Analytical Chemistry

Analytical Chromatography

Biochemicals

High-Purity Biochemicals and Reagents

Lab Chemicals

General description

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Formamide has been used:

as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Biochem/physiol Actions

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Features and Benefits

Versatile and adaptable for a wide variety of laboratory and research applications
BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

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pictograms

GHS08

signalword

Danger

hcodes

H351,H360D,H373

pcodes

P202 - P260 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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G. Wilkinson, ed.

In Situ Hybridization: A practical approach, 147-147 (1992)

R.C. Ogden et al.

Methods in Enzymology, 152, 67-67 (1987)

Single-tube nonaplex microsatellite PCR panel for preimplantation genetic diagnosis of Hb Bart's hydrops fetalis syndrome.

Min Chen et al.

Prenatal diagnosis, 35(6), 534-543 (2015-02-03)

To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel

Molecular Epidemiology of Mycoplasma pneumoniae: Genotyping Using Single Nucleotide Polymorphisms and SNaPshot Technology.

A Touati et al.

Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)

Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.

From formamide to purine: an energetically viable mechanistic reaction pathway.

Jing Wang et al.

The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)

A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest

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Global Trade Item Number

SKU	GTIN
47671-2.5L-F	04061832372662
47671-1L-F	04061832372655
47671-250ML-F	04061832372679

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