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Merck
CN

5006

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol™

Synonym(s):

Collagen Type I, PureCol Collagen

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About This Item

EC Number:
232-697-4
UNSPSC Code:
12352202
NACRES:
NA.75

Key Documents

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Product Name

PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

biological source

bovine

Quality Level

100

sterility

sterile-filtered

Assay

99.9%

form

lyophilized

packaging

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

Binding Specificity

Peptide Source: Elastin

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
8086
7682
7278

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Luisa Bracci et al.
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British journal of cancer, 121(2), 139-149 (2019-06-27)
Crenolanib is a tyrosine kinase inhibitor targeting PDGFR-α, PDGFR-β and Fms related tyrosine kinase-3 (FLT3) that is currently evaluated in several clinical trials. Although platelet-derived growth factor receptor (PDGFR) signalling pathway is believed to play an important role in angiogenesis
Michael Devos et al.
The Journal of experimental medicine, 217(7) (2020-04-22)
Aberrant detection of endogenous nucleic acids by the immune system can cause inflammatory disease. The scaffold function of the signaling kinase RIPK1 limits spontaneous activation of the nucleic acid sensor ZBP1. Consequently, loss of RIPK1 in keratinocytes induces ZBP1-dependent necroptosis
Rebecca Long Heise et al.
Tissue engineering. Part A, 15(12), 3951-3960 (2009-07-03)
Successful approaches to tissue engineering smooth muscle tissues utilize biodegradable scaffolds seeded with autologous cells. One common problem in using biological scaffolds specifically is the difficulty of inducing cellular penetration and controlling de novo extracellular matrix deposition/remodeling in vitro. Our

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