5006
Bovine Collagen Type I & III
from bovine hide, powder, suitable for cell culture, PureCol™
Synonym(s):
Collagen Type I, PureCol Collagen
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
Biological source:
bovine
Form:
lyophilized
Technique(s):
cell culture | mammalian: suitable
Assay:
99.9%
Product Name
PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture
biological source
bovine
Quality Segment
sterility
sterile-filtered
assay
99.9%
form
lyophilized
packaging
pkg of 15 mg
technique(s)
cell culture | mammalian: suitable
binding specificity
Peptide Source: Elastin
, Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
−20°C
General description
PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
Application
PureCol™ has been used:
- in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids
- to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
- to coat culture plates for collagen degradation assay with breast cancer cell lines
Preparation Note
This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.
Legal Information
Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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