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5006

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol™

Synonym(s):

Collagen Type I, PureCol Collagen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
Biological source:
bovine
Form:
lyophilized
Technique(s):
cell culture | mammalian: suitable
Assay:
99.9%
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Product Name

PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

biological source

bovine

Quality Level

100

sterility

sterile-filtered

assay

99.9%

form

lyophilized

packaging

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

binding specificity

Peptide Source: Elastin
, Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

−20°C

General description

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number
8086
7682
7278

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Articles

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3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

3D 细胞培养工具和技术

3D细胞培养概述。了解2D与3D细胞培养的对比结果，3D细胞培养的优势以及可用于开发3D细胞模型的技术

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Global Trade Item Number

SKUGTIN
14340-25MG04061838734808
14340-500MG04061838734815
14340-100MG04061838734792
14340-500M04061837383045
505875000104055977263114
5006-15MG04061832422558
HLTUD195604061836096236
HLTUD195504061836096229
HLMIR195504061836727635
HLMIR195604061836727642